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Thursday, June 13, 2024

DNA mobile

This pattern is for a mobile containing a DNA helix along with the 4 bases that make up DNA. It also contains the pattern to make Uracil (a base-like molecule found in RNA). 
DNA carries the genetic information that codes for all of the proteins that make up your body and allow it to function. The DNA model in the centre is approximately to scale, with a scale factor of 3x10^7. I've also included a lot of information on the science behind DNA intermixed with the pattern if you're interested.


Difficulty: 

Easy - medium


You will need:
  • A 2mm crochet hook
  • Dk yarn in purple, yellow, turquoise, dark pink, light pink, black, dark blue, and a scrap of white (although you're free to choose whatever colours do want for the base pairs)
  • An embroidery hoop
  • Craft wire
  • Black yarn or crochet cotton for the embroidery, I used black #10 crochet cotton (you could use embroidery thread, but you would need to use a full strand so it would work out pretty expensive)
  • Toy stuffing
  • A darning needle
  • A pair of scissors


Note:
  • 'sc12' means 'sc in each of the next 12sts'
  • 'fasten off neatly' refers to a special way of fastening off which is neater and makes it easier to see where you should be crocheting on the next round (also known as invisible fastening off). This involves pulling up your yarn through your stitch, threading the yarn underneath the second stitch from where you pulled your yarn up, then threading your yarn back through the top of this stitch and down through the back of the work. There is a very helpful site that explains it clearly found here. This is optional,but I find it makes the overall finish nicer.


So what is DNA?
DNA consists of 3 types of molecules: phosphate groups, a sugar called deoxyribose, and organic bases, which are what codes for all the proteins (see protein patterns) that make you up. These organic bases come in 4 different types, known as Cytosine, Adenine, Guanine, and Thymine, often referred to simply as C, A, G, and T respectively. 
These pair up within the structure of the DNA, with cytosine bonding with guanine, and adenine bonding with thymine. This is what ensures that DNA replication occurs correctly; if there is a C base on one DNA strand (see DNA helix pattern for more info on the structure of DNA), then there must be a G base on the other strand.




DNA II | Biology | Visionlearning

Part 1: The DNA bases


For Guanine:
Make all sections using turquoise yarn
For the smaller section (make 2):
Make a magic loop with 5sc
Round 1: make 2sc in each st (10sc)
Round 2: (sc, make 2sc in the next st) 5 times (15sc)
Round 3: (sc2, make 2sc in the next st) 5 times (20sc)
Round 4: (sc3, make 2sc in the next st) 5 times (25sc)
Round 5: sc5, ch6,  sc in the 2nd chain st from hook, sc in the next 4 chain sts, then sc in the same st as the one you crocheted in before you made the ch6, (sc4, make 2sc in the next st) 4 times
Round 6: sc4,  sc2tog between the next st and the back loop of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back of the last chain st. Make 2sc in the next st, sc3, sc2tog, (sc5, make 2sc in the next st) 4 times
Round 7: sc4, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog, sc5, make 2sc in the next st (sc6, make 2sc in the next st) twice, sc7
Fasten off neatly

For the front:
Make a magic loop with 6sc
Round 1: make 2sc in each st (12sc)
Round 2: (sc in the first st, make 2sc in the next st) 6 times to end (18sc)
Round 3: (sc in the first 2 sts, make 2sc in the next st) 6 times to end. ch1, turn (24sc)
Round 4: (sc in the first 3 sts, make 2sc in the next st) 6 times to end (30sc)
Round 5: sc4, make 2sc in the next st, (sc5, ch6,  sc in the 2nd chain st from hook, sc in the next 4 chain sts, then sc in the same st as the one you crocheted in before you made the ch6) twice, sc5,  ch11, sc in the 2nd loop from hook, sc in the next 4 chain sts, ch6, sc in the 2nd loop from hook, sc in the next 4 chain sts, sc in the next 5 chain sts of the ch11 section, then sc in the same st as the one you crocheted in before you made the ch11, (sc4, make 2sc in the next st) twice
Round 6: sc5, make 2sc in the next st, (sc4,  sc2tog between the next st and the back loop of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back of the last chain st. Make 2sc in the next st, sc3, sc2tog) twice, sc4, sc2tog between the next st and the back of the next chain st, sc in the back loop of the next 3 chain sts, sc2tog between the back loop of the next 2 chain sts, sc in the back loop of the next 3 chain sts. Make 2sc in the back of the last chain st, then make 2sc in the next st. Sc in the next 3 sts, sc2tog between the next st and the back of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back loop of the last chain st. Make 2sc in the next st, sc3, sc2tog, sc3, sc2tog, (sc5, make sc in the next st) twice
Round 7: sc6, make 2sc in the next st, sc4, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog, sc3, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog, sc3, sc2tog, sc2, sc2tog, sc3, make 2sc in the next st, slip6, sc4, make 2sc in the next st, slip9, sc2tog, sc5, make 2sc in the next st, sc6, make 2sc in the next st
Fasten off neatly

For the back:
Make a magic loop with 6sc
Round 1: make 2sc in each st (12sc)
Round 2: (sc in the first st, make 2sc in the next st) 6 times to end (18sc)
Round 3: (sc in the first 2 sts, make 2sc in the next st) 6 times to end. ch1, turn (24sc)
Round 4: (sc in the first 3 sts, make 2sc in the next st) 6 times to end (30sc)
Round 5: sc5, ch11, sc in the 2nd loop from hook, sc in the next 4 chain sts, ch6, sc in the 2nd loop from hook, sc in the next 4 chain sts, sc in the next 5 chain sts of the ch11 section, (sc5, ch6,  sc in the 2nd chain st from hook, sc in the next 4 chain sts, then sc in the same st as the one you crocheted in before you made the ch6) twice
Round 6: (sc4, make 2sc in the next st) 3 times, sc4, sc2tog between the next st and the back of the next chain st, sc in the back loop of the next 3 chain sts, sc2tog between the back loop of the next 2 chain sts, sc in the back loop of the next 3 chain sts. Make 2sc in the back of the last chain st, then make 2sc in the next st. Sc in the next 3 sts, sc2tog between the next st and the back of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back loop of the last chain st. Make 2sc in the next st, sc3, sc2tog, sc3, sc2tog, (sc4,  sc2tog between the next st and the back loop of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back of the last chain st. Make 2sc in the next st, sc3, sc2tog) twice, (sc5, make 2sc in the next st) 3 times
Round 7: sc4, sc2tog, sc2, sc2tog, sc3, make 2sc in the next st, slip6, sc4, make 2sc in the next st, slip9, sc2tog, (sc3, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog) twice, (sc5, make 2sc in the next st), sc6
Fasten off neatly

Place the front section next to one of the smaller sections, as shown in the first photo, and sew them together. Do the same for the back section, but make sure that the sections are lines up as shown in the third photo before you sew them together (so that they are the mirror image of the front).


After this you will need to embroider the chemical symbols onto each of the sections of crochet, as shown below (this shows the front section, you should embroider the reverse symbols onto the back section). The structure of each of the bases is also given at the start of this pattern as an extra reference point. Note that for bases G and A this structure shows the BACK of the the bases (this is important to allow them to pair up correctly)
 
I made each of the long straight sections representing the bonds by making a long strand from the start of the bond to the end, then making a few very small stitches along its length to secure it in place. This is demonstrated in the photos below.


You should also embroider the letter 'G' onto the back section.




For the eyes (make 2):
With black ch3
Round 1: make 2sc in the 2nd loop from hook, then make 3sc in the first chain st you made. Make 2sc into the back loop of the first chain st you crocheted into. (8sc)
Fasten off

Use white embroidery thread to embroider highlights onto the eyes, then sew them to the head. I found that the effect was better if you used the 'wrong side' of the crochet to face outwards. After you've done this embroider a mouth onto your base.


Sew the front and back sections of the head together , stuffing as you do so. Sew them together using a close backstitch (or running stitch if you prefer) so that you keep the ridge on the outside of the crochet.

Fun fact 1:
Typing at 60 words per minute for 8 hours a day it would take 50 years to type the entire human genome.


For Adenine:
Use yellow yarn throughout
For the small section (make 2):
Make a magic loop with 5sc
Round 1: make 2sc in each st (10sc)
Round 2: (sc, make 2sc in the next st) 5 times (15sc)
Round 3: (sc2, make 2sc in the next st) 5 times (20sc)
Round 4: (sc3, make 2sc in the next st) 5 times (25sc)
Round 5: sc5, ch6,  sc in the 2nd chain st from hook, sc in the next 4 chain sts, then sc in the same st as the one you crocheted in before you made the ch6, (sc4, make 2sc in the next st) 4 times
Round 6: sc4,  sc2tog between the next st and the back loop of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back of the last chain st. Make 2sc in the next st, sc3, sc2tog, (sc5, make 2sc in the next st) 4 times
Round 7: sc4, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog, sc5, make 2sc in the next st (sc6, make 2sc in the next st) 3 times, sc7
Fasten off neatly

For the larger section (make 2):
Make a magic loop with 6sc
Round 1: make 2sc in each st (12sc)
Round 2: (sc in the first st, make 2sc in the next st) 6 times to end (18sc)
Round 3: (sc in the first 2 sts, make 2sc in the next st) 6 times to end. ch1, turn (24sc)
Round 4: (sc in the first 3 sts, make 2sc in the next st) 6 times to end (30sc)
Round 5: sc5, ch11, sc in the 2nd loop from hook, sc in the next 4 chain sts, ch6, sc in the 2nd loop from hook, sc in the next 4 chain sts, sc in the next 5 chain sts of the ch11 section, then sc in the same st as the one you crocheted in before you made the ch11, (sc4, make 2sc in the next st) 5 times
Round 6: sc4, sc2tog between the next st and the back of the next chain st, sc in the back loop of the next 3 chain sts, sc2tog between the back loop of the next 2 chain sts, sc in the back loop of the next 3 chain sts. Make 2sc in the back of the last chain st, then make 2sc in the next st. Sc in the next 3 sts, sc2tog between the next st and the back of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back loop of the last chain st. Make 2sc in the next st, sc3, sc2tog, sc3, sc2tog, (sc5, make 2sc in the next st) 5 times
Round 7: sc4, sc2tog, sc2, sc2tog, sc3, make 2sc in the next st, slip6, sc4, make 2sc in the next st, slip9, sc2tog, (sc6, make 2sc in the next st) 3 times, sc7
Fasten off neatly

Once again you will need to sew each of the small sections to the larger sections, as shown below (this shows the front section: the back will be essentially the same but with the smaller section on the left and the larger section on the right)



You now need to embroider the chemical symbols in place. (It may be easier to use the image at the top of this pattern for the reference, since the photo below isn't ideal for showing whats going on.)
You should sew a letter 'A' onto the back section, similarly to how you did for guanine.



For the eyes (make 2):
With black ch3
Round 1: make 2sc in the 2nd loop from hook, then make 3sc in the first chain st you made. Make 2sc into the back loop of the first chain st you crocheted into.
Fasten off

Use white embroidery thread to embroider highlights onto the eyes, then sew them to the head, as you have did for guanine. Sew a smile onto the face.

You will then need to sew the front and back sections together, stuffing firmly.


Fun fact 2:
Every human shares 99.9% of their DNA with every other human



How do the bases know who they should pair up with?
The pairing of the bases occurs due to attractions called hydrogen bonds that occur between the bases. Essentially the hydrogen atoms on the outside of one base are attracted to the oxygen/ nitrogen atoms on the outside of the base its paired with. Cytosine and Guanine can form 3 of these bonds between each other, whilst  Adenine and Thymine can only form two. This is one factor that ensures they bond correctly.
Another factor is the shape the bases form when bonded together. As you may have noticed, the bases come in 2 different sizes. The smaller ones, Cytosine and Thymine, are known as pyrimidines, whereas Guanine and Adenine are classes as purines. The bonding of one pyrimidine with one purine endures that the overall structure formed has a very similar shape (as you should be able to see in the image below). This allows them to fit tightly together within the DNA strand. Any other bonding would not fit nicely within the DNA helix, meaning that it would be unstable, and would inevitably be ripped apart.

Additionally, this shape is able to fit neatly into the active site of the enzyme that creates DNA, known as DNA polymerase. (see enzyme pattern and possibly a pattern on DNA structure)




For Cytosine:
Use purple yarn throughout
For the front:
Make a magic loop with 6sc
Round 1: make 2sc in each st (12sc)
Round 2: (sc in the first st, make 2sc in the next st) 6 times to end (18sc)
Round 3: (sc in the first 2 sts, make 2sc in the next st) 6 times to end. ch1, turn (24sc)
Round 4: (sc in the first 3 sts, make 2sc in the next st) 6 times to end (30sc)
Round 5: sc5, ch11, sc in the 2nd loop from hook, sc in the next 4 chain sts, ch6, sc in the 2nd loop from hook, sc in the next 4 chain sts, sc in the next 5 chain sts of the ch11 section, then sc in the same st as the one you crocheted in before you made the ch11.(Sc4, make 2sc in the next st) twice, (sc5, ch6, sc in the 2nd chain st from hook, sc in the next 4 chain sts, then sc in the same st as the one you crocheted in before you made the ch6) twice, sc4, make 2sc in the next st
Round 6: sc4, sc2tog between the next st and the back of the next chain st, sc in the back loop of the next 3 chain sts, sc2tog between the back loop of the next 2 chain sts, sc in the back loop of the next 3 chain sts. Make 2sc in the back of the last chain st, then make 2sc in the next st (the first one you made when crocheting into the chain sts of the previous round). Sc in the next 3 sts, sc2tog between the next st and the back of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back loop of the last chain st. Make 2sc in the next st, sc3, sc2tog, sc3, sc2tog, sc5, make 2sc in the next st, sc5, make 2sc in the next st, (sc4, sc2tog between the next st and the back loop of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back of the last chain st. Make 2sc in the next st, sc3, sc2tog) twice, sc5, make 2sc in the next st
Round 7: sc4, sc2tog, sc2, sc2tog, sc3, make 2sc in the next st, slip6, sc4, make 2sc in the next st, slip9, sc2tog, sc5, make 2sc in the next st, sc6, make 2sc in the next st, sc4, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog, sc3,sc2tog, sc3, make 2sc in the next st, slip5, sc2tog, sc5, make 2sc in the following st, slip1
Fasten off neatly

For the back:
Make a magic loop with 6sc
Round 1: make 2sc in each st (12sc)
Round 2: (sc in the first st, make 2sc in the next st) 6 times to end (18sc)
Round 3: (sc in the first 2 sts, make 2sc in the next st) 6 times to end. ch1, turn (24sc)
Round 4: (sc in the first 3 sts, make 2sc in the next st) 6 times to end (30sc)
Round 5: sc5, ch11, sc in the 2nd loop from hook, sc in the next 4 chain sts, ch6, sc in the 2nd loop from hook, sc in the next 4 chain sts, sc in the next 5 chain sts of the ch11 section, then sc in the same st as the one you crocheted in before you made the ch11. sc4,  make 2sc in the next st,  (sc5, ch6, sc in the 2nd chain st from hook, sc in the next 4 chain sts, then sc in the same st as the one you crocheted in before you made the ch6) twice, (sc4, make 2sc in the next st) twice
Round 6: sc4, sc2tog between the next st and the back of the next chain st, sc in the back loop of the next 3 chain sts, sc2tog between the back loop of the next 2 chain sts, sc in the back loop of the next 3 chain sts. Make 2sc in the back of the last chain st, then make 2sc in the next st. Sc in the next 3 sts, sc2tog between the next st and the back of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back loop of the last chain st. Make 2sc in the next st, sc3, sc2tog, sc3, sc2tog, sc5, make 2sc in the next st, (sc4, sc2tog between the next st and the back loop of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back of the last chain st. Make 2sc in the next st, sc3, sc2tog) twice, (sc5, make 2sc in the next st) twice
Round 7: sc4, sc2tog, sc2, sc2tog, sc3, make 2sc in the next st, slip6, sc4, make 2sc in the next st, slip9, sc2tog sc5, make 2sc in the next st, sc4, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog, sc3, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog, (sc5, make 2sc in the next st) twice
Fasten off neatly

Embroider the chemical symbols onto each of the sections of crochet, as you have done for the previous bases, following the embroidery on the crochet below (which, once again, shows the front section of the crochet). You should also embroider the letter 'C' onto the back section, as shown below in the 2nd photo.


For the eyes (Make 2):
With black ch3
Round 1: make 2sc in the 2nd loop from hook, then make 3sc in the first chain st you made. Make 2sc into the back loop of the first chain st you crocheted into.
Fasten off

Use white embroidery thread to embroider highlights onto the eyes, then sew them to the head, as you have done for the previous bases


Sew the front and back sections of the bases together.



 




Fun fact 3:
Despite being much simpler organisms than humans, the gene of the aomeba is 200 times greater!

For Thymine:
Use dark pink yarn throughout
For the front and back (make 2):
Make a magic loop with 6sc
Round 1: make 2sc in each st (12sc)
Round 2: (sc in the first st, make 2sc in the next st) 6 times to end (18sc)
Round 3: (sc in the first 2 sts, make 2sc in the next st) 6 times to end. ch1, turn (24sc)
Round 4: (sc in the first 3 sts, make 2sc in the next st) 6 times to end (30sc)
Round 5: (sc5, ch6,  sc in the 2nd chain st from hook, sc in the next 4 chain sts, then sc in the same st as the one you crocheted in before you made the ch6) 5 times, sc4, make 2sc in the last st
Round 6: (sc4,  sc2tog between the next st and the back loop of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back of the last chain st. Make 2sc in the next st, sc3, sc2tog) 5 times, sc5, make 2sc in the last st
Round 7: sc4, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog, (sc3, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog) 4 times, sc5, make 2sc in the last st
Fasten off neatly

You now need to sew the chemical symbols onto the bases, as you did for the previous bases. The structure of thymine is shown below, (and can once again be found at the start of this pattern if you are struggling to see it properly). Once again you should sew the reverse of this structure onto the back of the base, and sew a 'T' into the centre, as shown below.


For the eyes (Make 2):
With black ch3
Round 1: make 2sc in the 2nd loop from hook, then make 3sc in the first chain st you made. Make 2sc into the back loop of the first chain st you crocheted into.
Fasten off

Embroider highlights onto these and sew them onto the face, as you did for cytosine. Embroider the mouth in place underneath, then sew the front and back sections together, stuffing firmly as you do so.





But wait - that's not all the possible bases
A similar molecule to our DNA is RNA. This is a single-stranded molecule whose strands differ in two ways to those of DNA: firstly the sugar in the structure is ribose, not deoxyribose, but also because the base Thymine is replaced with a slightly different base known as Uracil, the pattern for which is given below.


For Uracil:
Use light pink yarn throughout
For the front and back (make 2):
Make a magic loop with 6sc
Round 1: make 2sc in each st (12sc)
Round 2: (sc in the first st, make 2sc in the next st) 6 times to end (18sc)
Round 3: (sc in the first 2 sts, make 2sc in the next st) 6 times to end. ch1, turn (24sc)
Round 4: (sc in the first 3 sts, make 2sc in the next st) 6 times to end (30sc)
Round 5: (sc5, ch6,  sc in the 2nd chain st from hook, sc in the next 4 chain sts, then sc in the same st as the one you crocheted in before you made the ch6) 4 times, (sc4, make 2sc in the last st) twice
Round 6: (sc4,  sc2tog between the next st and the back loop of the next chain st, sc in the back loop of the next 3 chain sts, make 2sc in the back of the last chain st. Make 2sc in the next st, sc3, sc2tog) 4 times, (sc5, make 2sc in the last st) twice
Round 7: sc4, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog, (sc3, sc2tog, sc3, make 2sc in the next st, slip5, sc2tog) 3 times, (sc5, make 2sc in the last st) twice
Fasten off neatly

You now need to sew the chemical symbols onto the bases. These are exactly the same as for Thymine, but without the single protruding line that symbolises a CH3 group (as you can see below)






For the eyes (Make 2):
With black ch3
Round 1: make 2sc in the 2nd loop from hook, then make 3sc in the first chain st you made. Make 2sc into the back loop of the first chain st you crocheted into.
Fasten off


Embroider highlights onto these and sew them onto the face, as you did for the previous bases. Embroider the mouth in place underneath, then sew the front and back sections together, stuffing firmly as you do so.




But why is Uracil present in RNA instead of Thymine?
When life first started, the genetic code was stored in  RNA instead of DNA. However, this was inherently unstable, because of the presence of the OH group of the 2' position on the ribose sugar (link to another pattern that explains this further). When organisms became more complex RNA was replaced with DNA, which consisted of deoxyribose, in which this OH group was missing. Another form of instability came from the fact that certain mutations in DNA can result in cytosine bases undergoing a chemical process that changed them into uracil bases. Luckily, our clever cells evolved a DNA repair enzyme called DNA glycosylase  (see enzyme pattern for more information on enzymes) to pick up on this and convert these mutated bases back into cytosine. This is why it is necessary that uracil is replaced with thymine, which has a very similar structure (so is still able to bind to adenine), but is not exactly the same. If uracil were present the DNA repair enzymes would go around converting all the uracil bases it found into cytosine, regardless of whether or not this is what they should actually be.



Part 2: The DNA helix

For the sugar-phosphate backbone (make 2):
With dark blue dk yarn ch9
Round 1: sc in the first chain st you made, then sc in every following chain st to make a loop
Round 2: sc in each of these 9 sts
Repeat round 2 until the backbone measures around 28cm
Fasten off 

Information on the DNA backbone 
This backbone of the DNA helix is made up of alternating molecules of phosphate and a sugar called deoxyribose. It is a strong structure that is not easily broken. The backbone strands have a polarity. This is different from the type of polarity you may have encountered in chemistry, but it gives us a way to specify the direction of the molecule. This polarity is determined by the direction of the deoxyribose molecules, and to see how this works we need to first examine the structure of these and how they fit together with the phosphate groups in the chain.

The deoxyribose sugar contains 5 carbon atoms, and they are numbered 1 - 5, with the first carbon atom being the one attached to the bases, and the 5th one being the side chain attached to the phosphate group. This means that the 3rd carbon atom will also be attached to a phosphate group. Therefore the DNA backbone is said to run from the 5' end (from the phosphate group attached to a carbon number 5) to the 3' end (i.e. the phosphate group attached to a carbon number 3). This gives the polarity of the strand. In DNA the two backbone strands run in opposite directions relative to each other, and this has important implications during processes such as DNA replication.

For the bases:
You need to make a total of 40 bases, and the number of G and C bases should be equal, as should the number of A and T bases.

With a 2mm hook make a magic loop with 5sc
Round 1 - 3: (3 rounds) sc in each st
Fasten of

To make up:
Sew the bases together in pairs (A with T, C with G)
Thread a length of wire through the backbone, and sew it to the edge so that it is held in place. This will keep the backbone rigid, so that the helix holds its shape better.

You will then need to sew the bases in place. Start by sewing one base pair to the bottom of the helix and another to the top, to ensure that the two backbone strands will match up. 

After this you need to sew the remaining base pairs in between. Try to twist the helix as you sew them on, to ensure that it will keep its helix shape. You should try to get between 1.5 and 2 full turns into the length of the helix (technically it should be 2 turns but I found that this was very difficult to achieve).

The final stage is to assemble the mobile. Cut 3 lengths of white yarn approx. 25cm long and tie them at equal intervals to the edge of the embroidery hoop. Tie the other ends to each other in the centre of the loop. Secure the ends in place with a dab of PVA glue.



Next you need to sew another section of yarn to the centre of the hoop, to where you tied the three lengths of yarn together. Sew the other end of this to the helix, so that the centre of the nucleus hangs about 25cm below the embroidery hoop. You should then attach the cuddly bases around the outside so that they hang at varying lengths, as shown below.


That's all for this pattern! I hope you have found it interesting. As I said I plan to make a whole series of biochemistry-related patterns - please let me know if there's anything particular you'd like me to make. This applies especially to if there was anything mentioned in this post that you were unsure about or thought wasn't explained properly - I'm planning to do some patterns going into more detail about some of the topics mentioned. 

Please let me know if you have any questions about this or any of my other patterns, either by leaving a comment below or via my Ravelry or Instagram pages.
You are very welcome to sell finished products but I do ask that you don't re-post this pattern or claim it as you're own. Thank you!


References:

Sunday, September 3, 2023

Pelvis assembly

This pattern is part of my life-sized anatomically correct skeleton series and includes details on how to make the ligaments around the pelvis, and how to attach the hip and leg bones together. I have included a large number of photographs to show the positioning of the various ligaments.



Difficulty:

Easy - Medium


You will need:


Use this diagram for reference when sewing ligaments to the innominate bone

For the pubic symphysis:
ch31
Round 1: sc in the first chain st you made, then sc in each of the following chain sts to make a loop (31sc)
Rounds 2 - 3: (2 rounds) sc in each st
Fasten off

Sew each side of this cylinder to the symphysis pubis part of each of the innominate bone, as shown below


You now need to attach the legs to the innominate bones


For the pubofemoral ligament:
ch23
Row 1: sc in the 2nd chain st from hook and the next 21sts. ch1, turn (22sc)
Rows 2 - 3: (2 rows) sc in each st. ch1, turn
Row 4: hdc6, sc16. ch1, turn
Row 5: sc16, hdc6. ch1, turn
Row 6: hdc6, sc16. ch1, turn
Row 7: slip14, sc2, hdc6. ch1, turn
Row 8: hdc3, sc, slip1
Fasten off

Sew the thinner edge of this to the lower side of the head of the femur, and the thicker end to the innominate, with the side with the hdc sts on closest to the pubic symphysis section.

For the iliofemoral liagment:
For the first section:
ch23
Row 1: sc in the 2nd chain st from hook and the next 21sts. ch1, turn (22sc)
Row 2: hdc11, sc11. ch1, turn
Row 3: sc11, hdc11. ch1, turn
Row 4: hdc11, sc11. ch1, turn
Row 5: sc11, hdc11. ch1, turn
Row 6: hdc11, sc11. ch1, turn
Row 7: sc11, hdc11. ch1, turn
Row 8: hdc11, sc11. ch1, turn
Row 9: sc11, hdc11. ch1, turn
Rows 10 - 12: (3 rows) sc in each st. ch1, turn
Row 13: sc in each st
Fasten off

Sew the thicker edge of this to the femur, just above where you attached the previous ligament. The side with the hdc sts should be at the bottom. Sew the other end of this ligament to the inominate bone, above where the previous ligament was attached. The ligament should be at an angle to the pubofemoral liagment, as shown in the images below.



Turn the pelvis over so that the back of the joint is facing upwards

For the upper section:
ch23
Row 1: sc in the 2nd chain st from hook and the next 21sts. ch1, turn (22sc)
Rows 2 - 17: (16 rows) sc in each st. ch1, turn
Row 18: sc in each st
Fasten off

Sew this to the top of the joint, as shown below (not that the 4th image shows a top-down view of the bone)




For the lower section:
ch31
Row 1: sc in the 2nd chain st from hook and the next 29sts. ch1, turn (30sc)
Row 2: make 2sc in the first st, sc27, sc2tog. ch1, turn (30sc)
Row 3: sc2tog, sc27, make 2sc in the last st. ch1, turn
Row 4: make 2sc in the first st, sc27, sc2tog. ch1, turn
Row 5: sc2tog, sc27, make 2sc in the last st. ch1, turn
Row 6: make 2sc in the first st, sc27, sc2tog. ch1, turn
Row 7: sc2tog, sc28. ch1, turn (29sc)
Row 8: sc27, sc2tog. ch1, turn (28sc)
Row 9: sc2tog, sc26 (27sc)
Fasten off
Don't sew in place yet


For the isciofemoral ligament:
ch27
Row 1: sc in the 2nd chain st from hook and the next 25sts. ch1, turn (26sc)
Row 2: hdc13, sc13. ch1, turn
Row 3: slip11, sc4, hdc11. ch1, turn
Row 4: hdc11, sc4, slip11. ch1, turn
Row 5: sc13, hdc12, make 2hdc in the last st. ch1, turn (27sts)
Row 6: hdc11, sc5, slip11. ch1, turn
Row 7: slip11, sc5, hdc11. ch1, turn
Row 8: make 2hdc in the first st, hdc12, sc12, sc2tog. ch1, turn (27sts)
Row 9: slip11, sc5, hdc11. ch1, turn
Row 10: hdc11, sc5, slip11. ch1, turn
Row 11: sc2tog, sc13, hdc11, make 2hdc in the last st. ch1, turn (27sts)
Row 12: hdc11, sc14, sc2tog. ch1, turn (26sts)
Row 13: sc2tog, sc12, hdc12. ch1, turn (25sts)
Row 14: make 2hdc in the first st, hdc10, sc12, sc2tog (25sts)
Fasten off

Sew the thicker edge of this to the innominate bone, so that the cast-on end is at the top (see image). Sew the other edge of this ligament to the femur so that it ovelaps with the upper iliofemoral ligament section.



Finally sew the lower iliofemoral liagment in place. It should be positioned so that one end covers the gap between the lower and middle iliofemoral ligament, and the other wraps around to cover the gap between the lower iliofemoral liagment and the isciofemoral liagment. Note that the cast-on/off ends should be between the lower iliofemoral liagment and the isciofemoral liagment.
(front of joint before ligament attached)


The same process then needs to be repeated for the left femur, but the attatchment of the ligaments is mirrored. This can be seen in the images below.







Now you need to attach the inominate bones to the sacrum. The following images show the positioning of the sacrum with respect the the innominate bones from different angles. This should help you get the placement right then attaching the ligaments.




For the anterior sacroiliac ligament:
This is formed from a number of narrow bands that attach the sacrum and inominate bones on the anterior side. However, in this pattern these bands have been combined into 2 sections

ch16
Row 1: sc in the 2nd chain st from hook and the next 14sts. ch1, turn (15sc)
Row 2: hdc8, sc6, make 2sc in the last st. ch1, turn (16sts)
Row 3: make 2sc in the first st, sc6, hdc9. ch1, turn (17sts)
Row 4: hdc2tog, hdc6, slip9. ch1, turn (16sts)
Row 5: slip8, sc3, hdc3, hdc2tog. ch1, turn (15sts)
Row 6: hdc2tog, hdc4, sc8, make 2sc in the last st. ch1, turn (15sts)
Row 7: slip5, sc4, hdc4, hdc2tog. ch1, turn (14sts)
Row 8: hdc2tog, hdc4, sc4, slip4. ch1, turn (13sts)
Row 9: make 2sc in the first st, sc6, hdc4, hdc2tog. ch1, turn (13sts)
Row 10: hdc2tog. hdc3, sc5, slip3. ch1, turn (12sts)
Row 11: slip3, sc3, hdc4, hdc2tog. ch1, turn (11sts)
Row 12: hdc2tog, hdc, sc4, slip4. ch1, turn (10sts)
Row 13: sc6, hdc2, hdc2tog. ch1, turn (9sts)
Row 14: hdc2tog, hdc2, sc2, slip3. ch1, turn (8sts)
Row 15: make 2sc in the first st, slip2, sc2, hdc3. ch1, turn (9sts)
Row 16: sc8, make 2sc in the last st. ch1, turn (10sts)
Row 17: make 2sc in the first st, sc4, hdc5. ch1, turn (11sts)
Row 18: hdc5, sc, slip5. ch1, turn
Row 19: sc in each st. ch1, turn
Row 20: sc  in each st
Fasten off

Sew this to the inner edge of this to the sacrum. It should be sewn so that it curves around the upper edge of the sacrum, around where the lowest intervertebral disk is attached (assuming you've attached the spine already). This can be seen in the images below.


ch16
Row 1: sc in the 2nd chain st from hook and the next 14sts. ch1, turn (15sc)
Rows 2 - 10: (9 rows) sc in each st. ch1, turn
Row 11: sc2tog, sc13 (14sc)
Row 12: sc12, sc2tog (13sc)
Fasten off

Sew this to the sacrum just below where you attached the previous ligament. Make sure to attach the edge opposite to the one where you fastened off, and that the cast-off edge is in the lowest corner, The grain of the rows should run parallel to the gain of the rows of the sacrum that you sew it over.


Position the sacrum  next to the innominate bone. This can be seen in the above images, and on this website https://www.healthline.com/human-body-maps/sacroiliac-ligament#1 (reduce the layers down so you can only see the bones)

Sew the opposite end of the 2 ligaments attached to the sacrum to the innominate bones, as shown in the photos below. You should ideally find that the 2nd of these ligaments tucks under the first, as shown in the images.



Repeat this for the left-hand side


For the sacrospinous ligament:
ch13
Row 1: hdc in the 2nd chain st from hook and the next 4 chain sts, sc3, slip4. ch1, turn
Row 2: slip4, sc3, hdc5. ch1, turn
Row 3: hdc5, sc3, slip4. ch1, turn
Row 4: slip4, sc3, hdc5. ch1, turn
Row 5: hdc2tog, hdc3, sc3, slip4. ch1, turn
Row 6: slip4, sc3, hdc2, hdc2tog
Fasten off

Sew the thinner edge of this to the ischial body of the inominate bone.


Sew the other, thicker end to the sacrum/coxxyx, so that the loose end created when starting off is a little way down the coxxyx (see images below). Repeat this for the left-hand side.


For the iliolumbar liagment:
This consists of a series of ligament sections, all made individually

ch8
Row 1: slip the 2nd chain st from hook and the next 2 chain sts. sc4. Ch1, turn (7sts)
Row 2: sc4, slip3, ch1, turn
Row 3: slip3, sc4. ch1, turn
Row 4: sc4, slip3, ch1, turn
Row 5: slip3, sc4. ch1, turn
Row 6: sc4, slip3
Fasten off

Sew the thicker end of this to the SAFs of the L5 vertebra. Sew the other end to the innominate bone, as shown in the images.


Repeat this for the left-hand side


ch21
Row 1: sc in the 2nd chain st from hook and the next 19sts. ch1, turn (20sc)
Row 2: make 2sc in the first st, sc7, slip12. ch1, turn (21sts)
Row 3: slip12, sc8, make 2sc in the last st. ch1, turn (22sts)
Row 4: make 2sc in the first st, sc9, slip13. ch1, turn (23sts)
Row 5: slip13, sc10, make 2sc in the last st (24sts)
Fasten off

Sew the thinner end of this to the  SAF of the L5 vertebra, so that it overlaps with the lower side of where you attached the previous ligament section. Sew the other end to the innominate bone, around the region of the greater sciatic notch, as shown below.



ch8
Row 1: sc in the 2nd chain st from hook and the next 6sts. ch1, turn (7sc)
Row 2: make 2sc in the first st, sc4, sc2tog. ch1, turn
Row 3: sc2tog, sc4, make 2sc in the last st. ch1, turn
Row 4: make 2sc in the first st, sc4, sc2tog. ch1, turn
Row 5: sc2tog, sc4, make 2sc in the last st. ch1, turn
Row 6: make 2sc in the first st, sc4, sc2tog
Fasten off
Sew one end of this to the base of the SAF of L4, and the other end to the top of the first iliolumbar section you made.



Turn the pelvis over

For the back of the iliolumbar liagment:
ch8
Row 1: slip the 2nd chain st from hook and the next 2 chain sts. sc4. Ch1, turn (7sts)
Row 2: sc4, slip3, ch1, turn
Row 3: slip3, sc4. ch1, turn
Row 4: sc4, slip3, ch1, turn
Row 5: slip3, sc4. ch1, turn
Row 6: sc4, slip3
Fasten off

Sew the thinner end of this to the back of the SAF of L5, and sew the thicker end to the innominate bone, in approximately the same place as you sewed the first part of the iliolumar ligament on the front.



For the posterior sacroiliac ligament:
This also consists of a large number of individual ligament sections

ch15
Row 1: sc in the 2nd chain st from hook and the next 13sts. ch1, turn (14sc)
Row 2: sc2tog, sc11, make 2sc in the last st. ch1, turn
Row 3: make 2sc in the first st, sc11, sc2tog. ch1, turn
Row 4: sc2tog, sc11, make 2sc in the last st. ch1, turn
Row 5: make 2sc in the first st, sc11, sc2tog. ch1, turn
Row 6: sc2tog, sc11, make 2sc in the last st
Fasten off

Sew this to the pelvis, with one end being sewn to the side of the central divide of the sacrum, and the other end being sewn to the front side of the innominate, just below where you sewed the iliolumbar ligament.



ch8
Row 1: sc in the 2nd chain st from hook and the next 6sts. ch1, turn (7sc)
Row 2: make 2sc in the first st, sc4, sc2tog. ch1, turn
Row 3: sc2tog, sc4, make 2sc in the last st. ch1, turn
Row 4: make 2sc in the first st, sc4, sc2tog
Fasten off

Sew one side of this section to the sacrum, just to the side of the top sacral foramen (hole). Sew the other side the the back of the innominate, just underneath where the previous ligament was sewn.


ch14
Row 1: hdc in the 2nd chain st from hook and the next 2 chain sts, sc10. ch1, turn (13sts)
Row 2: sc10, hdc3. ch1, turn
Row 3: hdc3, sc10. ch1, turn
Row 4: sc10, hdc3. ch1, turn
Row 5: hdc3, sc10
Fasten off

Sew this underneath the previous ligament, as shown in the photo. The thicker edge should be sewn to the edge of the central section of the sacrum.

ch11
Row 1: sc in the 2nd chain st from hook and the next 9sts. ch1, turn (10sc)
Rows 2 - 4: (3 rows) sc in each st. ch1, turn
Row 5: sc in each st
Fasten off

Sew this just below the previous ligament, as shown below




ch16
Row 1: hdc in the 2nd chain st from hook, then hdc in the next 2 chain sts, sc12. ch1, turn
Row 2: sc12, hdc3. ch1, turn
Row 3: hdc3, sc12. ch1, turn
Row 4: sc12, hdc3
Fasten off

Sew this underneath the previous ligament. The thinner end should be sewn to the innominate so that is ovelaps with the previous ligament you attached.




ch15
Row 1: sc in the 2nd chain st from hook and the next 13sts. ch1, turn (14sc)
Rows 2 - 4: (3 rows) sc in each st. ch1, turn
Row 5: sc in each st
Fasten off

This ligament is attached slightly differently from the previous ones. It should be sewn at an angle, so that one end touches the end of the innominate, and the other end resides beside the bottom sacral foramen., as shown below





ch9
Row 1: hdc in the 2nd chain st from hook and the next 2 chain sts, sc2, slip3. ch1, turn
Row 2: slip3, sc2, hdc3. ch1, turn
Row 3: hdc3, sc2, slip3. ch1, turn
Row 4: slip3, sc2, hdc3
Fasten off

Sew  the thicker end of this to the edge of the central part of the sacrum in between the last 2 sacral foramen. Sew the other end of this to the previous ligament you attached.


ch9
Row 1: hdc in the 2nd chain st from hook and the next 2 chain sts, sc2, slip3. ch1, turn
Row 2: slip3, sc2, hdc3. ch1, turn
Row 3: hdc3, sc2, slip3. ch1, turn
Row 4: slip3, sc2, hdc3
Fasten off

Sew this underneath the bottom sacral foramen, as shown below.

Repeat this on the left-hand side, mirroring the attachment of the ligaments.





For the sacrotuberous ligament (make 2):
ch53
Row 1: hdc in the 2nd loop from hook and the next 2 chain sts, sc in the next 49 chain sts. ch1, turn (52sts)
Row 2: sc49, hdc2, make 2hdc in the last st. ch1, turn (53sts)
Row 3: make 2hdc in the first st, hdc3, sc49. ch1, turn (54sts)
Row 4: sc27, ch7, sc in the 2nd chain st from hook, then sc in the next 5 chain sts. sc in the next 22sts,  hdc5. ch1, turn (60sts)
Row 5: hdc5, sc28, make 3sc in the back of the chain st you first crocheted into when crocheting down the chain. sc in the back of the next 5 chain sts, sc27 (68sts)
Row 6: slip22, sc3, hdc3tog, hdc3, sc, slip4, sc, hdc3, hdc3tog, sc3, slip 22 (64sts)
Fasten off

Sew to the pelvis, as shown in the images below.




Turn the skeleton back over so the front is facing you

For the inguinal ligament (make 2):
ch34
Row 1: sc in the 2nd chain st from hook and the next 32sts. ch1, turn (33sc)
Fasten off

Sew to the innominate between the anterior superior iliac spine and the ischium, as shown in the image below




That's all from this pattern - I hope you've enjoyed it! If you have any questions please feel free to contact me at lhodginscrochet@gmail.com and I'll do my best to answer them. As always you're very welcome to sell finished products but please don't re-post this pattern or claim it as your own. Thank you!